The present manuscript describes simple, accurate, precise and economical dual wavelength and derivative spectrophotometric method for estimation of minoxidil and aminexil in their combined topical solution. Minoxidil and aminexil in combination is used as hair growth stimulant in medical condition of androgenic alopecia. The dual wavelength method describes determination of minoxidil by the absorbance difference between two wavelengths 286.98 nm and 271.66 nm and aminexil by the absorbance difference between 301.35 nm and 290.33 nm. The first order derivative method describes the determination of minoxidil at ZCP of aminexil 296.10 nm and of aminexil at ZCP of minoxidil 280 nm. The linearity was observed in the concentration range of 4-20 μg/ml and 3-15 μg/ml for minoxidil and aminexil respectively for both the methods. These methods were successfully applied for assay of marketed formulation. The suitability of these methods for the quantitative determination of minoxidil and aminexil was proved by recovery study and other validation parameters. The proposed methods were found to be simple and sensitive for the routine quality control analysis of minoxidil and aminexil in their combined topical solutions.
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